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In vivo examination of membrane protein localization and degradation with green fluorescent protein
R Y Hampton1, A Koning, R Wright
1Department of Molecular and Cell Biology, University of California, Berkeley 94720, USA.
Abstract:
To test the utility of green fluorescent protein (GFP) as an in vivo reporter protein when fused to a membrane domain, we made a fusion protein between yeast hydroxymethylglutaryl-CoA reductase and GFP. Fusion proteins displayed spatial localization and regulated degradation consistent with the native hydroxymethylglutaryl-CoA reductase proteins. Thus, GFP should be useful in the study of both membrane protein localization and protein degradation in vivo.