[Methods for the preparation of a pure soluble antigen from Toxoplasma gondii]

In this study we report 4 methods for separating leucocytes from toxoplasma found in peritoneal exudate of infected mice. a) Passage through No: 26 needle, b) glass adherence, c) passage through glass wool, d) sonic vibration. Experiment showed that sonic vibration at 60 watt; 60 seconds was the best way to destroy selectively almost all leucocytes present in the preparation. In order to prepare a soluble antigen from clean toxoplasmas sonic vibration at 70 watt, 60 second was found sufficient to destroy all parasites present in the suspension. We suggest that this pure T. gondii antigen, may give more reliable results in passive hemagglutination, precipitation and complement fixation tests.