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Flow cytometry analysis of the neutralization effect of anti-IL-8 McAbs on IL-8-activated human granulocytes

Y Y Ji1, G Q Ye

  • 1Shanghai Institute of Cell Biology, Academia Sinica, China.

Shi Yan Sheng Wu Xue Bao
|September 1, 1995
PubMed

Two hybridoma clones, I8-S2 and I 8-63, secreting anti- IL-8 antibodies were constructed from fusion of immunized spleen cells of BALB/c mice with either Sp 2/0 or 653 myeloma cells. They recognized different epitopes on IL-8 molecule. IL-8 could activate human granulocytes and induce the elevation of [Ca2+]i. Through flow cytometry analysis the two clones displayed different neutralizing effects on the cell activation function of IL-8. The neutralizing effect of clone I8-S2 was apparently higher than that of clone I8-63. It is suggested that the cell activation function of IL-8 is restricted to a certain epitope of IL-8 molecule.

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