Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[Relationship between CENP-B gene expression and the cell cycle]

H X Zhang1, D G Chen, A Peng

  • 1Key Lab of Cell Proliferation and Regulation Biology, Beijing Normal University.

Shi Yan Sheng Wu Xue Bao
|September 1, 1995
PubMed
Summary

Centromere Protein B (CENP-B) is expressed throughout the cell cycle, with highest levels in G2 phase. This continuous expression is crucial for centromere/kinetochore assembly and function during cell proliferation.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Combined transcriptome and metabolome analyses reveal that galactose metabolism is vital for tobacco in response to low-nitrate stress.

Journal of genetics·2026
Same author

[Preliminary development of a competency evaluation framework for experts in investigator-initiated trials quality assessment].

Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine]·2026
Same author

[FGFR1 mRNA expression in different molecular subtypes of breast cancer].

Zhonghua bing li xue za zhi = Chinese journal of pathology·2026
Same author

[Association between lifestyle and life expectancy in adults in Henan Province].

Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi·2026
Same author

[HER2 protein expression and gene status in endometrial serous carcinoma].

Zhonghua bing li xue za zhi = Chinese journal of pathology·2026
Same author

[Prevalence and influencing factors of clinical obesity and preclinical obesity among rural adults].

Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi·2025

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Genetics

Context:

  • The centromere/kinetochore is vital for chromosome segregation during cell division.
  • Centromere Protein B (CENP-B) is a key component of this structure.
  • Understanding CENP-B's role in the cell cycle is essential for comprehending cell proliferation.

Purpose:

  • To investigate the cell cycle-dependent expression pattern of CENP-B.
  • To explore the relationship between CENP-B gene expression and cell proliferation.
  • To examine the association of CENP-B with the nuclear skeleton during different cell cycle phases.

Summary:

  • CENP-B gene is expressed throughout the cell cycle in HeLa cells, with peak expression in G2 phase and lowest in S phase.
  • Immunostaining revealed distinct centromere/kinetochore structures across G1, S, and G2 phases.

Related Experiment Videos

  • Southern blot analysis indicated a stronger association of CENP-B with the nuclear skeleton in G2 phase compared to S phase.

    • Impact:

    • Findings suggest continuous CENP-B expression is necessary for timely centromere/kinetochore assembly and function.
    • This study provides insights into the regulation of centromere proteins during cell division.
    • The results contribute to understanding the molecular mechanisms underlying chromosome segregation and cell proliferation.