Related Experiment Videos
Stimulation of nuclear polyphosphoinositide synthesis by GTP-gamma-S: a potential regulatory role for nuclear
A M Martelli1, R Bareggi, L Cocco
1Dipartimento di Morfologia Umana Normale, Trieste, Italy.
Biochemical and Biophysical Research Communications
|January 5, 1996
Abstract:
The nonhydrolyzable GTP analogue GTP-gamma-S was capable of stimulating in vitro phosphorylation of polyphosphoinositides in isolated nuclei prepared from mouse erythroleukemia cells. On the contrary, GDP-beta-S was ineffective. The stimulation was not detectable when nuclei were prepared from erythroleukemia cells induced to differentiate by exposure to dimethyl sulfoxide. Both nuclear phosphomonoesterase and phospholipase C activities were not influenced by GTP-gamma-S. Our results point to the likelihood that nuclear phosphoinositide kinases might be regulated by a GTP-binding protein.