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Cyclin D1 protein expression in mantle cell lymphoma
S Alkan1, B Schnitzer, J L Thompson
1Department of Pathology, University of South Florida, H. Lee Moffitt Cancer Center, Tampa, USA.
Summary
Cyclin D1 protein overexpression is a key indicator for mantle cell lymphoma. This finding helps distinguish it from chronic lymphocytic leukemia, aiding in accurate diagnosis and treatment strategies for lymphoproliferative disorders.
Area of Science:
- Hematology
- Oncology
- Molecular Biology
Background:
- The t(11;14) chromosomal translocation is linked to mantle cell lymphomas and other lymphoproliferative disorders.
- This translocation involves the bcl-1 oncogene and leads to elevated cyclin D1 mRNA levels in malignant B cells.
Purpose of the Study:
- To investigate cyclin D1 protein expression in various low-grade lymphoproliferative disorders.
- To assess the utility of cyclin D1 protein analysis in differentiating mantle cell lymphoma from chronic lymphocytic leukemia.
Main Methods:
- Western blot analysis was employed to examine cyclin D1 protein expression.
- The study included 33 patients with low-grade lymphoproliferative disorders and 2 with reactive hyperplasias.
- Cytogenetic and immunophenotypic analyses were also performed on select cases.
Main Results:
- Cyclin D1 overexpression was detected in 8 out of 11 mantle cell lymphomas.
- No cyclin D1 overexpression was observed in chronic lymphocytic leukemia, hairy cell leukemia, Sezary syndrome, monocytoid B-cell lymphomas, follicular lymphomas, or reactive hyperplasias.
- Mantle cell lymphomas and chronic lymphocytic leukemias exhibited coexpression of CD5 and CD20.
Conclusions:
- Mantle cell lymphoma can be morphologically and immunophenotypically similar to chronic lymphocytic leukemia.
- Cyclin D1 protein expression analysis is a valuable tool for distinguishing mantle cell lymphoma from other low-grade lymphoproliferative disorders.