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Related Experiment Videos

A comprehensive hematologic study in calves with total artificial hearts

H Harasaki1, K Fukamachi, M Benavides

  • 1Department of Biomedical Engineering, Cleveland Clinic Foundation, Ohio 44195, USA.

ASAIO Journal (American Society for Artificial Internal Organs : 1992)
|July 1, 1995
PubMed
Summary

Investigating complications of artificial hearts in calves, this study found that while some coagulation assays are usable, others are not. Hematocrit decreased slightly, and platelet aggregation was temporarily suppressed, with low rates of infection and thromboembolism.

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Area of Science:

  • Biomedical Engineering
  • Hematology
  • Immunology

Background:

  • Thromboembolism and infection are significant risks associated with long-term circulatory assist devices.
  • Device implantation can alter the hemostatic system and blood cell functions, increasing complication susceptibility.
  • Assessing these alterations requires reliable immunologic and coagulation assays, particularly in animal models.

Purpose of the Study:

  • To evaluate the utility of various coagulation and flow cytometry assays in calves for studying device-related complications.
  • To assess hematologic and hemostatic changes in calves implanted with a total artificial heart.
  • To determine the incidence of infection and thromboembolism in this animal model.

Main Methods:

  • In vitro testing of coagulation assays (TAT, TxB2, GPIIbIIIa, aminophospholipid) and flow cytometry in calves.

Related Experiment Videos

  • Measurement of hematocrit, platelet aggregation, and polymorphonuclear cell functions in calves with a total artificial heart.
  • Monitoring for device infection and thromboembolism over the study period.

    • Main Results:

    • Thromboxane B2 (TxB2), platelet surface glycoprotein IIbIIIa (GPIIbIIIa), and membrane aminophospholipid assays were usable in calves; others like F1.2 and D-dimer were not.
    • A sustained mild decrease in hematocrit was observed, attributed to increased blood volume, not red blood cell damage.
    • Platelet aggregation was suppressed for 3 days post-surgery with decreased GPIIbIIIa expression; immune cell functions remained unaltered.

    Conclusions:

    • Specific coagulation assays are viable for calf studies, aiding research into circulatory assist devices.
    • Total artificial heart implantation in calves causes transient hematologic and platelet function changes without significant immune cell alteration.
    • The incidence of infection and thromboembolism was low in this calf model, suggesting potential for future research.