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Related Experiment Videos

Newly designed hemostatic technology based on photocurable gelatin

Y Nakayama1, T Matsuda

  • 1Department of Bioengineering, National Cardiovascular Center Research Institute, Osaka, Japan.

ASAIO Journal (American Society for Artificial Internal Organs : 1992)
|July 1, 1995
PubMed
Summary

This study introduces a new light-activated hemostatic glue using photocurable gelatins and xanthene dyes. This innovative technology rapidly stops bleeding and promotes tissue regeneration after liver injury.

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Area of Science:

  • Biomaterials Science
  • Surgical Technology
  • Photochemistry

Background:

  • Hemostatic agents are crucial for controlling bleeding during surgery.
  • Existing hemostatic methods have limitations in speed and efficacy.
  • Photochemically activated materials offer potential for rapid and targeted hemostasis.

Purpose of the Study:

  • To develop and evaluate a novel photochemically driven hemostatic technology.
  • To assess the efficacy of a photocurable gelatin-based hemostatic glue in achieving hemostasis and promoting tissue repair.
  • To demonstrate the in situ application of the hemostatic glue during laparoscopic surgery.

Main Methods:

  • Synthesis of photocurable gelatins derivatized with xanthene dyes (fluorescein, eosin, rose bengal).

Related Experiment Videos

  • Formulation of a hemostatic glue comprising dye-derivatized gelatin, poly(ethylene glycol) diacrylate, and ascorbic acid.
  • In vivo testing on a rat liver injury model, involving application of the glue and visible light irradiation.
  • Histological analysis of the injured liver region at 7 days post-surgery.
  • Demonstration of in situ gelation during simulated laparoscopic surgery using a fiberscope.

    • Main Results:

    • Visible light irradiation rapidly converted the hemostatic glue into a swollen gel, achieving hemostasis within seconds.
    • Increased irradiation time enhanced gel yield and reduced water swellability.
    • Histological examination revealed minimal remaining gelatin, good tissue regeneration, and limited necrosis at 7 days post-surgery.
    • Successful in situ gelation and hemostasis were demonstrated during simulated laparoscopic procedures.

    Conclusions:

    • The developed photochemically driven hemostatic glue is effective in achieving rapid hemostasis and promoting tissue regeneration.
    • This technology shows promise for minimally invasive surgical applications, including laparoscopic procedures.
    • The dye-sensitized photo-crosslinking and photograft polymerization offer a controllable and efficient method for hemostatic agent activation.