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Involvement of class A calcium channels in the KCl induced Ca2+ influx in hippocampal synaptosomes
J O Malva1, A F Ambrósio, A P Carvalho
1Department of Zoology, University of Coimbra, Portugal.
Abstract:
Using Ca2+ channel toxins, we determined the types of voltage-sensitive calcium channels activated by two levels of KCl depolarization in hippocampal synaptosomes. The increase in the intracellular Ca2+ concentration ([Ca2+]i) induced by 30 mM KCl was equally sensitive to either omega-agatoxin IVA (omega-Aga IVA) or to omega-conotoxin MVIIC (omega-CgTx MVIIC), and the inhibition produced by these two peptides was not additive. The present results indicate that omega-Aga IVA and omega-CgTx MVIIC do not distinguish between two different VSCC in hippocampal synaptosomes and that they both inhibit a channel with the alpha 1A subunit which is present in the rat hippocampus.