Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Crystallization and structure determination of RNA

S E Lietzke1, C L Barnes, C E Kundrot

  • 1Department of Chemistry and Biochemistry, University of Colorado Boulder 80309-0215, USA.

Current Opinion in Structural Biology
|October 1, 1995
PubMed
Summary

Recent advances in RNA synthesis, purification, and crystallization have enabled the determination of multiple X-ray crystal structures. Future methods promise further insights into the structural basis of RNA functions.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Characterization of HKE2: an ancient antigen encoded in the major histocompatibility complex.

Tissue antigens·2007
Same author

Which strategy for a protein crystallization project?

Cellular and molecular life sciences : CMLS·2004
Same author

Reaction of [Os(en)(2)O(2)](2+) with photolyzed [Mo(CN)(8)](4-) in water.

Inorganic chemistry·2001
Same author

Functionalization of [MoW(5)O(19)](2-) with aromatic amines: synthesis of the first arylimido derivatives of mixed-metal polyoxometalates.

Inorganic chemistry·2001
Same author

Synthesis of some 2'- and 3'-fluoroalkyl substituted nucleosides and oligonucleotides.

Nucleosides, nucleotides & nucleic acids·2001
Same author

Generation of cyclopentadienones from 2-bromocyclopentenones.

The Journal of organic chemistry·2001

Area of Science:

  • Structural Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Ribonucleic acid (RNA) plays critical roles in gene expression and regulation.
  • Understanding RNA structure is essential for deciphering its diverse biological functions.

Purpose of the Study:

  • To highlight recent progress in determining RNA crystal structures.
  • To discuss the implications of these structures for understanding RNA function.

Main Methods:

  • X-ray crystallography techniques applied to purified RNA molecules.
  • Development of novel methods for RNA synthesis, purification, and crystallization.

Main Results:

  • Several high-resolution X-ray crystal structures of RNA molecules have been determined.

Related Experiment Videos

  • These structures provide atomic-level details of RNA architecture.

    • Conclusions:

    • Advances in structural determination techniques are rapidly expanding our knowledge of RNA.
    • Future structural studies will further elucidate the functional mechanisms of RNA molecules.