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Updated: Aug 18, 2026

Preparation of Synaptoneurosomes from Mouse Cortex using a Discontinuous Percoll-Sucrose Density Gradient
Published on: September 17, 2011
Postnatal development of the calcium-dependency of glutamate release from rat cortical synaptosomes: comparison with
1Physiology Unit School of Molecular and Medical Biosciences University of Wales, Cardiff, UK.
Abstract:
The ontogeny of the Ca(2+)-dependency of the depolarisation-induced release of preloaded [3H]glutamate and [3H]5-hydroxytryptamine [5-HT] from rat cortical synaptosomes was examined. 5-HT release was found to be exclusively Ca(2+)-dependent at all ages studied. In contrast, glutamate release only showed a significant Ca(2+)-dependent component from postnatal day 10 [PND 10] onwards. This correlated with the ontogeny of the glutamate accumulating activity of synaptic vesicles, a finding consistent with vesicles being the site of Ca(2+)-dependent release. The effectiveness of K(+)-depolarisation in inducing the Ca(2+)-dependent release of both transmitters increased during the early neonatal period, reaching near adult levels at PND20 for 5-HT and PND30 for glutamate.
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