Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

The cellular effects of E2F overexpression

P D Adams1, W G Kaelin

  • 1Dana-Farber Cancer Institute, Boston, MA 02115, USA.

Current Topics in Microbiology and Immunology
|January 1, 1996
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Structural Biology Resources at an Upgrading Synchrotron Advanced Light Source.

Synchrotron radiation news·2026
Same author

ALS-ENABLE: creating synergy and opportunity at the Advanced Light Source synchrotron structural biology beamlines.

Journal of synchrotron radiation·2025
Same author

Bulk-solvent and overall scaling revisited: faster calculations, improved results. Corrigendum.

Acta crystallographica. Section D, Structural biology·2023
Same author

The role of aging in cancer.

Molecular oncology·2022
Same author

AKT overactivation can suppress DNA repair via p70S6 kinase-dependent downregulation of MRE11.

Oncogene·2017
Same author

ChIP-Sequencing to Map the Epigenome of Senescent Cells Using Benzonase Endonuclease.

Methods in enzymology·2016
Same journal

Resolution Failure in Periodontal Diseases: Dysregulated Pro-resolving Mechanisms in Chronic Inflammation and Tissue Breakdown.

Current topics in microbiology and immunology·2026
Same journal

Correction to: Resolution of Skeletal Muscle Inflammation: Role of Specialized Pro-resolving Lipid Mediators in the Recovery from Exercise, Injury, and Disease.

Current topics in microbiology and immunology·2026
Same journal

Resolution Biology in Soft Tissue Joint Disease.

Current topics in microbiology and immunology·2026
Same journal

A 25+ Year Journey on Yeast-Regulated Cell Death Research.

Current topics in microbiology and immunology·2026
Same journal

Adoptive T-Cell Immunotherapy.

Current topics in microbiology and immunology·2026
Same journal

Resolution Pharmacology Targeting the Melanocortin System.

Current topics in microbiology and immunology·2026
See all related articles

The retinoblastoma protein (pRB) controls cell cycle progression via E2F transcription factors. Dysregulation of pRB and E2F proteins can promote cancer development.

Area of Science:

  • Molecular Biology
  • Cell Cycle Regulation
  • Cancer Biology

Background:

  • The retinoblastoma tumor-suppressor gene (RB) produces pRB, a nuclear phosphoprotein regulating cell cycle G1/S phase transition.
  • pRB's tumor-suppressive function is linked to its negative regulation of cellular proliferation.
  • The E2F family of transcription factors are key mediators of pRB's growth-inhibitory effects.

Purpose of the Study:

  • To review insights into the mechanism of action of E2F family members.
  • To explore the regulatory relationship between pRB, p130, p107, and E2F family members.
  • To discuss how pRB and E2F inactivation may cooperate in tumorigenesis.

Main Methods:

  • Overexpression studies of E2F family members.
  • Studies in RB-/- SAOS-2 cells to investigate pRB and p130 regulation of E2F.

Related Experiment Videos

  • Analysis of cell cycle arrest override and apoptosis induction by E2F1 overexpression.

    • Main Results:

    • Evidence supports pRB and p130 negatively regulating E2F family members.
    • E2F1 is primarily regulated by pRB, and E2F4 by p130.
    • E2F1 overexpression can override cell cycle arrest but leads to p53-dependent apoptosis.

    Conclusions:

    • Functional inactivation of pRB and p53 may cooperate in promoting tumorigenesis.
    • E2F family members possess oncogenic potential under certain conditions.
    • These proteins play a critical role in controlling cellular proliferation.