Related Experiment Videos
Application of PCR for detection of toxigenic Corynebacterium diphtheriae strains isolated during the Russian
V M Mikhailovich1, V G Melnikov, I K Mazurova
1Russian Federal Reference Laboratory for Diphtheria, Gabrichevsky Institute of Epidemiology and Microbiology, Moscow, Russia.
Journal of Clinical Microbiology
|November 1, 1995
Abstract:
A total of 250 Corynebacterium diphtheriae isolates from clinical cases and carriers in Russia were assayed by PCR directed at the A subunit of the diphtheria toxin gene to distinguish toxigenic from nontoxigenic strains; 170 strains were positive as indicated by the presence of the 248-bp amplicon. The results of this PCR assay were in complete concordance with those of the standard immunoprecipitation assay (Elek), and the PCR assay is a useful tool for rapid identification in clinical laboratories.