5'-nucleotidase activity as a synaptic marker of parasagittal compartmentation in the mouse cerebellum

In the molecular layer of the mouse cerebellum, the histochemical activity of the adenosine-producing ectoenzyme 5'-nucleotidase discloses a parasagittal pattern of alternating enzyme-rich and enzyme-poor bands. In the rat, 5'-nucleotidase activity transiently labels cerebellar synapses during postnatal development and shifts later on towards an exclusive glial location in the molecular layer. We therefore asked whether different ultrastructural expression of 5'-nucleotidase would account for the light microscopic pattern seen in the adult mouse cerebellum. Using an enzyme cytochemical method, we localized 5'-nucleotidase activity on the glial cells and at the main types of asymmetrical synapses in the developing and mature cerebellum of the mouse. The percentage of labelled synapses increased until adulthood within the 5'-nucleotidase-positive bands. Here, the vast majority (86%) of the synapses were labelled against only 27% within the negative bands in the adult. Thus, 5'-nucleotidase appears as a marker of glia and of Purkinje cell synapses across cerebellar compartments. Changes in purinergic neuromodulation and/or cell adhesion mediated by 5'-nucleotidase across bands might participate in the functional differentiation of the cerebellar parasagittal subsets.