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Related Experiment Videos

Interplay between mycoplasmas and host target cells

J B Baseman1, M Lange, N L Criscimagna

  • 1Department of Microbiology, University of Texas Health Science Center at San Antonio 78284-7758, USA.

Microbial Pathogenesis
|August 1, 1995
PubMed
Summary
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Mycoplasmas, including Mycoplasma penetrans, Mycoplasma pneumoniae, and Mycoplasma genitalium, actively infect mammalian cells, residing both on surfaces and within intracellular spaces for over a week. This challenges previous understandings of their role in host cells.

Area of Science:

  • Microbiology
  • Cell Biology
  • Infectious Diseases

Background:

  • Mycoplasmas are common intracellular pathogens.
  • Their intracellular infectious patterns are not well understood.

Purpose of the Study:

  • To investigate the infectious pattern of mycoplasmas in mammalian cells.
  • To determine the intracellular persistence and localization of mycoplasmas.

Main Methods:

  • Confocal microscopy
  • Flow cytometry
  • Cell fractionation
  • Mycoplasma viability assays

Main Results:

  • Mycoplasmas were observed on cell surfaces within 2 hours post-infection.
  • Mycoplasmas entered intracellular spaces, localizing in cytoplasmic and perinuclear regions.

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  • Mycoplasmas persisted intracellularly for at least 7 days, cultivable from nuclear and cytoplasmic fractions.

    • Conclusions:

    • Mycoplasmas exhibit a more active intracellular role than previously recognized.
    • Intracellular persistence suggests novel mechanisms of host-cell interaction and potential pathogenesis.