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Detection of astroviruses from stool samples in Japan using reverse transcription and polymerase chain reaction
K Saito1, H Ushijima, O Nishio
1Department of Microbiology, Institute of Public Health, Tokyo, Japan.
Microbiology and Immunology
|January 1, 1995
Abstract:
We developed a reverse transcription and polymerase chain reaction (RT-PCR) method for detecting astrovirus serotypes 1, 2, 3, 5, 6 and 7 (but not serotype 4). Furthermore, we developed the specific primers for detecting serotypes 1 and 2, the most predominant serotypes in the world. Sensitivity of the first PCR with serotype common primers was about 10 times higher than that of enzyme immunoassay with monoclonal antibody (EIA-MAb). Sensitivity of the second PCR with the serotype-specific primers was even higher. The RT-PCR method was useful for detecting astroviruses from clinical samples, especially serotypes 1 and 2.