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Protein-protein interactions in eukaryotic transcription initiation: structure of the preinitiation complex
1Department of Chemistry, Rutgers University, New Brunswick, NJ 08855, USA.
Abstract:
We have used alanine scanning to analyze protein-protein interactions by human TATA-element binding protein (TBP) within the transcription preinitiation complex. The results indicate that TBP interacts with RNA polymerase II and general transcription factors IIA, IIB, and IIF within the functional transcription preinitiation complex and define the determinants of TBP for each of these interactions. The results permit construction of a model for the structure of the preinitiation complex.