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Cell-context signalling

R I Pennell1, Q C Cronk, L S Forsberg

  • 1Department of Biology, University College London, U.K.

Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences
|October 30, 1995
PubMed
Summary
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Plant cell position is regulated by cell collectives expressing specific polysaccharide epitopes. The JIM8 epitope is crucial for somatic embryo development and meristematic activity, influencing plant growth and development.

Area of Science:

  • Plant developmental biology
  • Cell biology
  • Molecular biology

Background:

  • Plant cell differentiation is influenced by neighboring cells.
  • Proteoglycan antigens, identified by monoclonal antibody (MAb) probes, signify cellular position.
  • These antigens are present on the surfaces of all plant cells.

Purpose of the Study:

  • To investigate the role of cell collectives defined by polysaccharide epitopes in plant development.
  • To understand the regulatory function of cell-context in early plant development.
  • To identify specific cell-cell interactions crucial for somatic embryogenesis.

Main Methods:

  • Sorting of carrot somatic cells based on polysaccharide epitope expression using MAbs.
  • Culturing embryo initial cells in altered contextual backgrounds.

Related Experiment Videos

  • Analyzing the effect of JIM8-reactive cell collectives on somatic embryo development.
  • Investigating signal transfer from JIM8-reactive cells in carrot seedlings.

    • Main Results:

    • Specific cell collectives induce and sustain early embryo development.
    • Somatic embryo initials require the JIM8-reactive cell collective for proper development; absence leads to callus formation.
    • The JIM8 epitope is necessary for sustaining meristematic activity.
    • Out-of-context signals from JIM8-reactive cells stimulate lateral root production in seedlings.
    • Similar cell-cell interactions are observed in normal flower development.

    Conclusions:

    • Cell collectives defined by polysaccharide epitopes play a vital regulatory role in early plant development.
    • The JIM8 epitope-defined cell collective is essential for initiating and sustaining meristematic activity for somatic embryogenesis.
    • Cell-cell communication involving the JIM8 epitope is a key interaction in carrot development, from embryogenesis to seedling stages.