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[Investigations on egg yolk-free diluting medium for the cryopreservation of bull spermatozoa]
F Müller-Schlösser1, E Hinsch, J Böhm
1Zentralbesamungsstation Giessen eG.
Abstract:
On the basis of various laboratory researches and field experiments with 959 alternating first inseminations the traditional tris-extender and a yolk-free medium for semen freezing on soy lecithin base were compared in studies using a split-sample technique. Concerning important criteria such as non-return-rate, conservation quality, and standard of hygiene and practicability, the alternative product was found at least of equal quality. The renunciation of chicken yolk is to be seen as a general advantage. Here, the development of cryomedia has been enriched by a useful and sensitive new component. Apart from being competitive this product is also the first to conform to future demands in standardization through ISO-norm.