Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Cross-complexing pattern of plant histones

S Spiker, I Isenberg

|May 3, 1977

Summary

Pea histones H2a and H2b show significant differences from mammalian counterparts, yet maintain similar interaction patterns. This suggests conserved histone-histone binding despite plant-specific structural variations.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Evidence against an effect of plant hormones on thermal denaturation of pea nucleoprotein.

Planta·2014

Same author

Constancy of wheat histones during development.

Planta·2014

Same author

Blotting Index of Dissimilarity: use to study immunological relatedness of plant and animal High Mobility Group (HMG) chromosomal proteins.

Plant molecular biology·2013

Same author

Chromosomal proteins of Arabidopsis thaliana.

Plant molecular biology·2013

Same author

The role of cell differentiation state and HMG-I/Y in the expression of transgenes flanked by matrix attachment regions.

Transgenic research·2001

Same author

Increasing loop domain size does not diminish effects of matrix attachment regions on transgene expression in tobacco cells culture.

FEBS letters·2001

Area of Science:

Biochemistry
Molecular Biology
Plant Science

Background:

Histones are crucial proteins for DNA packaging in eukaryotic cells.
Plant histones, particularly H2a and H2b, exhibit structural variations compared to mammalian histones.

Purpose of the Study:

To investigate the interactions of pea histones (H2a, H2b, H3, H4) and compare them to calf thymus histones.
To determine if structural differences in pea histones affect their interaction patterns.

Main Methods:

Isolation of pea histones H2a, H2b, H3, and H4.
Study of histone interactions using fluorescence anisotropy, light scatter, and circular dichroism.

Main Results:

Pea H2b has a higher molecular weight and different amino acid composition than calf thymus H2b.
Pea H2a exists as two molecular weight species, unlike calf thymus H2a.
Despite structural differences, pea histones exhibit conserved strong interactions (H3-H4, H2b-H4, H2a-H2b) and similar weaker interactions as calf histones.

Conclusions:

Plant histones H2a and H2b display significant molecular and compositional differences from their mammalian counterparts.
The cross-complexing patterns of pea histones are conserved and similar to those of calf thymus histones.
Conserved histone-histone interactions suggest functional similarities despite evolutionary divergence in histone structure.

Related Experiment Videos