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Infection model for hatching chicks infected with Salmonella enteritidis
U Methner1, S al-Shabibi, H Meyer
1Federal Institute for Health Protection of Consumers and Veterinary Medicine, Jena, Germany.
Summary
Artificial infection of eggs with Salmonella enteritidis (phage type 4) caused rapid embryonic death via yolk infection. Albumen inoculation led to hatching of infected chicks, modeling vertical transmission for pathogen-host studies.
Area of Science:
- Veterinary Microbiology
- Food Safety
- Avian Pathology
Background:
- Salmonella enteritidis is a significant foodborne pathogen.
- Vertical transmission of Salmonella in poultry poses a challenge to food safety.
- Understanding Salmonella pathogenesis in ovo is crucial for control strategies.
Purpose of the Study:
- To establish an artificial infection model for Salmonella enteritidis in hatching eggs.
- To investigate the effects of Salmonella enteritidis infection on embryonic development and hatchability.
- To explore the potential for vertical transmission of Salmonella enteritidis in poultry.
Main Methods:
- Artificial inoculation of six Salmonella enteritidis (phage type 4) strains into egg yolk and albumen of SPF hatching eggs.
- Assessment of Salmonella multiplication, embryonic mortality, and hatchability.
- Microbiological analysis to confirm Salmonella presence in hatched chicks.
Main Results:
- Low-dose yolk infection with S. enteritidis resulted in rapid Salmonella multiplication and embryonic death.
- Albumen inoculation with S. enteritidis led to the hatching of infected day-old chicks.
- The study successfully modeled primary vertical transmission of Salmonella.
Conclusions:
- Artificial infection models are effective for studying Salmonella enteritidis pathogenesis in ovo.
- Vertical transmission of Salmonella can occur through albumen inoculation, impacting hatchling health.
- This model provides a valuable tool for investigating Salmonella-host interactions in poultry.