Related Experiment Video
Updated: Oct 7, 2026

Ex Vivo Imaging of Postnatal Cerebellar Granule Cell Migration Using Confocal Macroscopy
Published on: May 12, 2015
Activation of large conductance Ca(2+)-activated K+ channels in N1E-115 neuroblastoma cells by platelet-activating
M Diserbo1, M Fatôme, J Verdetti
1Centre de Recherches Emile Pardé, La Tronche, France.
Abstract:
Using patch-clamp single channel recording techniques, we reported a Platelet-Activating Factor "PAF"-induced activation of large conductance Ca(2+)-activated K+ "BK(Ca)" channels in N1E-115 cells. This activation was only observed in cell-attached configuration and was blocked by the PAF antagonist BN50739 or removal of calcium from the bath. Nanomolar concentration of PAF produced a transient hyperpolarization observed in whole-cell current clamp configuration which was blocked by the bath application of BN50739 or iberiotoxin. Our results suggest that the PAF-induced hyperpolarization is mediated by an activation of BK(Ca) channels coupled to specific PAF receptors. This coupling is not direct and results from the PAF-induced elevation in cytosolic free Ca2+ concentrations that we have previously described in N1E-115 cells (Cell Calcium 1995, 17, 442-452).
More Related Videos
11:47Real-time Live-cell Flow Cytometry to Investigate Calcium Influx, Pore Formation, and Phagocytosis by P2X7 Receptors in Adult Neural Progenitor Cells
Published on: April 3, 2019
04:37Comprehensive Analysis of Procoagulant Platelets Exhibiting Features of Necrosis, Apoptosis and Platelet Activation
Published on: May 23, 2025
Related Concept Videos
Enzyme-linked Receptors
MAPK Signaling Cascades
cAMP-dependent Protein Kinase Pathways
Feedback Regulation of Calcium Concentration
Various transmembrane receptors, such as G protein-coupled receptors (GPCRs), elicit a response to extracellular signals by increasing cytosolic calcium. Activated GPCRs...
Calmodulin-dependent Signaling
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...
Formation of the Platelet Plug
As the injured blood vessel contracts, endothelial cells undergo contraction, revealing collagen fibers in the basement membrane and underlying connective tissue. Furthermore, the plasma membrane of endothelial cells becomes adhesive, preparing the site for platelet adhesion. Platelets...