[Influence of cultivation temperature on blast-transformation of lymphocytes from humans of different ages]

Studies on the effect of temperature in tissue culture upon the phytohemagglutinin blast-transformation of lymphocytes obtained from persons aged 20 to 35 and 90 to 102 years were carried out. The tissue culture temperature of 39 degrees C increased the blast-transformation index as compared with control (37 degrees C), whereas the temperature of 41 degrees C sharply decreased it. The effect of temperature upon the blast-transformation process in tissue culture had some peculiarities depending on donor's age.