Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Assessing bone quantity by pQCT

J A Gasser1

  • 1Sandoz Pharma Ltd., Basel, Switzerland.

Bone
|October 1, 1995
PubMed
Summary
This summary is machine-generated.

Peripheral quantitative computed tomography (pQCT) offers a sensitive and reproducible method for monitoring bone changes in small animals. This non-invasive technique is valuable for drug evaluation and basic research, detecting bone loss or gain faster than DEXA.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Therapy for musculoskeletal disorders.

Journal of orthopaedic translation·2018
Same author

Protein malnutrition attenuates bone anabolic response to PTH in female rats.

Endocrinology·2014
Same author

Effects of 3 years treatment with once-yearly zoledronic acid on the kinetics of bone matrix maturation in osteoporotic patients.

Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·2012
Same author

Bone degeneration and recovery after early and late bisphosphonate treatment of ovariectomized wistar rats assessed by in vivo micro-computed tomography.

Calcified tissue international·2008
Same author

Coupled or uncoupled remodeling, is that the question?

Journal of musculoskeletal & neuronal interactions·2006
Same author

Bone anabolic agents. Introduction.

Journal of musculoskeletal & neuronal interactions·2005

Area of Science:

  • Biomedical Engineering
  • Osteoporosis Research
  • Pharmacology

Background:

  • Assessing bone loss and gain in animal models is crucial for understanding osteoporosis and evaluating bone-building therapies.
  • Existing methods like DEXA may lack the sensitivity or speed required for early detection of bone changes in small animal studies.
  • Peripheral quantitative computed tomography (pQCT) offers high-resolution imaging, but its application in small animals for dynamic bone monitoring needed further validation.

Purpose of the Study:

  • To evaluate the efficacy of the XCT960A pQCT system for detecting bone loss in ovariectomized (OVX) rats and bone gain in rats treated with human parathyroid hormone (hPTH(1-34)).
  • To determine an optimal site on the proximal rat tibia for sensitive and reproducible bone parameter measurements.
  • To compare the sensitivity and speed of pQCT with DEXA for monitoring bone changes in small animal models.

Related Experiment Videos

Main Methods:

  • In vivo testing of the XCT960A pQCT system on OVX rats and hPTH(1-34) treated rats.
  • Repositioning of animals to assess measurement reproducibility (in vivo precision).
  • Identification and validation of an optimal measurement site on the proximal tibia (5mm distal to the knee joint).

Main Results:

  • High precision was achieved with in vivo coefficient of variation (CV) values ranging from 1.6% to 5.9%.
  • Significant changes in tibial bone parameters were detected as early as 2 weeks post-OVX or hPTH(1-34) treatment.
  • Optimal measurement site identified 5mm distal to the knee joint, offering reduced partial volume effects and suitability for histomorphometry comparison.

Conclusions:

  • pQCT is a sensitive, reproducible, and non-invasive method for monitoring bone mass, density, and geometric properties in small animals like rats.
  • pQCT is more sensitive than DEXA for detecting early bone changes and allows for shorter experimental durations, making it valuable for drug evaluation.
  • pQCT serves as a complementary technique to histomorphometry, enhancing the capabilities for basic research and therapeutic assessment in bone studies.