Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Acyclic oligonucleotide analogues

Y Merle1, E Bonneil, L Merle

  • 1URA 500 du CNRS, Faculté des Sciences de Rouen, Mont-Saint-Aignan, France.

International Journal of Biological Macromolecules
|October 1, 1995
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

SUMOylation- and GAR1-Dependent Regulation of Dyskerin Nuclear and Subnuclear Localization.

Molecular and cellular biology·2021
Same author

[Identification of proteins associated with transcription factors HOXA9 and E2A-PBX1 by tandem affinity purification].

Molekuliarnaia biologiia·2017
Same author

Proteogenomic-based discovery of minor histocompatibility antigens with suitable features for immunotherapy of hematologic cancers.

Leukemia·2016
Same author

Potentially inappropriate drug prescription in the elderly in France: a population-based study from the French National Insurance Healthcare system.

European journal of clinical pharmacology·2011
Same author

Hepatitis B vaccine and the risk of CNS inflammatory demyelination in childhood.

Neurology·2009
Same author

Inappropriate medications in the elderly.

Clinical pharmacology and therapeutics·2008
Same journal

Cellulose-based MnO<sub>2</sub> nanocomposites for the efficient adsorption of copper (II) ions from aqueous solutions.

International journal of biological macromolecules·2026
Same journal

Efficient isolation of cellulose from Arundo donax L. using deep eutectic solvent for the preparation of regenerated fiber.

International journal of biological macromolecules·2026
Same journal

Proteomic characterization of cellular responses to the fish allergen β-parvalbumin using ZenoSWATH-MS.

International journal of biological macromolecules·2026
Same journal

Lignin-based macromolecular composite solid electrolyte enabling high-performance sodium metal batteries via MgF<sub>2</sub>-assisted ion dissociation.

International journal of biological macromolecules·2026
Same journal

In situ self-growth nano-selenium-loaded peptidoglycan-based biomimetic bioparticles as a versatile immunoregulatory delivery platform for subunit vaccines.

International journal of biological macromolecules·2026
Same journal

Targeted depolymerization of waste cotton textiles mediated by deep eutectic solvents: High-value recycling of waste cotton textiles into regenerated fibers.

International journal of biological macromolecules·2026
See all related articles

Acyclic nucleotide analogues show stability against enzymatic degradation and can function as primers for DNA synthesis. These synthetic oligonucleotides form stable duplexes, offering potential in biochemical research.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Synthetic Chemistry

Background:

  • Oligonucleotides are crucial in molecular biology, but natural forms are susceptible to enzymatic degradation.
  • Developing stable oligonucleotide analogues is important for various applications, including diagnostics and therapeutics.

Purpose of the Study:

  • To synthesize and characterize acyclic analogues of oligothymidylate and oligoadenylate.
  • To evaluate their thermal stability, resistance to snake venom phosphodiesterase, and primer/template properties with DNA polymerase.

Main Methods:

  • Synthesis of acyclic oligothymidylate and oligoadenylate analogues.
  • Thermal melting studies (Tm) to assess duplex stability.
  • Enzymatic assays using snake venom phosphodiesterase and Klenow fragment of DNA polymerase I.

Related Experiment Videos

  • Circular dichroism spectroscopy.
  • Molecular modeling.

    • Main Results:

    • Acyclic dodecaadenylate ((GlyA)12) formed a stable duplex with dodecathymidylate (p(dT)12), exhibiting A-conformation characteristics.
    • (GlyA)12 demonstrated high stability against snake venom phosphodiesterase, over 10,000 times slower hydrolysis than p(dT)12.
    • (GlyA)12 functioned as a primer for Klenow DNA polymerase, albeit with slower polymerization rates compared to natural primers.

    Conclusions:

    • Acyclic oligonucleotide analogues possess enhanced stability and can participate in DNA synthesis.
    • The synthesized analogues are promising candidates for further investigation in nucleic acid research and biotechnology.
    • A-conformation is an energetically feasible structure for these synthetic duplexes.