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Comparison of four methods for forage nitrate analysis

C L Bedwell1, D W Hamar, M L Hoesterey

  • 1Diagnostic Laboratory, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Ft. Collins 80523, USA.

Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
|October 1, 1995
PubMed
Summary

The nitrate-selective electrode method offers superior accuracy and precision for analyzing nitrate levels in forage samples compared to other tested methods. This analytical technique demonstrates the lowest variation and highest reliability for feed analysis.

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Area of Science:

  • Agricultural Science
  • Analytical Chemistry

Background:

  • Accurate nitrate quantification in forage is crucial for animal nutrition and health.
  • Existing analytical methods for nitrate determination vary in accuracy and precision.

Purpose of the Study:

  • To compare the accuracy and precision of four different methods for analyzing nitrate content in forage.
  • To identify the most reliable method for nitrate determination in feed samples.

Main Methods:

  • Four analytical methods were employed: diphenylamine spot plate, spectrophotometric, nitrate-selective electrode, and high-performance liquid chromatography.
  • Twenty forage samples with varying nitrate levels were analyzed, alongside spiked feed extracts.

Main Results:

  • The nitrate-selective electrode method exhibited the lowest coefficient of variation (6.4%) and highest accuracy, with recoveries near 100%.

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  • Spectrophotometric and nitrate-selective electrode methods showed similar percent recoveries.
  • The diphenylamine spot plate method displayed the poorest recovery and greatest variation.

    • Conclusions:

    • The nitrate-selective electrode method is recommended as the most accurate and precise technique for forage nitrate analysis.
    • This finding supports improved feed quality assessment and animal health management through reliable nitrate testing.