Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

T-cell development and selection in the intestinal epithelium

P Poussier1, M Julius

  • 1Wellesley Hospital Research Institute, Toronto, Canada.

Seminars in Immunology
|October 1, 1995
PubMed
Summary

T cell development in the gut is complex. While some T cells mature identically to those in the thymus, others show unique gut-specific development and selection processes.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A review on diatom biosilicification and their adaptive ability to uptake other metals into their frustules for potential application in bone repair.

Journal of materials chemistry. B·2021
Same author

A comparison of the degradation behaviour of 3D printed PDLGA scaffolds incorporating bioglass or biosilica.

Materials science & engineering. C, Materials for biological applications·2021
Same author

Filament extrusion of bioresorbable PDLGA for additive manufacturing utilising diatom biosilica to inhibit process-induced thermal degradation.

Journal of the mechanical behavior of biomedical materials·2021
Same author

Dysregulated liver lipid metabolism and innate immunity associated with hepatic steatosis in neonatal BBdp rats and NOD mice.

Scientific reports·2019
Same author

An orthologous non-MHC locus in rats and mice is linked to CD4<sup>+</sup> and CD8<sup>+</sup> T-cell proportion.

Genes and immunity·2017
Same author

Genetic dissection of Iddm26 in the spontaneously diabetic BBDP rat.

Genes and immunity·2014

Area of Science:

  • Immunology
  • Gastroenterology
  • Cell Biology

Background:

  • T lymphocyte (T cell) development in the murine intestinal epithelium is established but poorly understood.
  • Key aspects of T cell lymphopoiesis in the gut, including in situ development of specific T cell receptor (TCR) alpha beta+ intraepithelial lymphocyte (IEL) subsets, remain controversial.

Purpose of the Study:

  • To investigate the in situ development and selection of TCR alpha beta+ IEL subsets in the murine intestinal epithelium.
  • To compare T cell development and selection in the gut with traditional thymic T cell development.
  • To explore the mechanisms underlying the oligoclonal repertoire of TCR alpha beta+ IEL.

Main Methods:

  • Analysis of athymic radiation chimeras and parabionts to study IEL development and selection.
  • Utilizing transgenic TCR alpha beta models restricted to MHC class I and MHC class II.
  • Employing two model systems to investigate the basis of the oligoclonal IEL repertoire, including comparing IEL development in orthotopic vs. ectopic intestines and using mice lacking Lck.

Main Results:

  • TCR alpha beta+ IEL development and selection processes in the gut can differ from thymic development.
  • While MHC class I-restricted TCR alpha beta+ IEL development yields a functional repertoire identical to thymic T cells, MHC class II-restricted TCR alpha beta+ IEL development shows distinct features.
  • Distinct mechanisms regulate T cell development in the gut, with evidence suggesting roles for luminal antigen and TCR alpha beta-mediated signals in shaping the IEL repertoire.

Conclusions:

  • The murine intestinal epithelium supports unique T cell development and selection pathways.
  • Gut-specific features, particularly related to MHC class II molecules on enterocytes, influence T cell development.
  • Further research using specific models is crucial to fully elucidate the mechanisms governing IEL repertoire formation.

Related Experiment Videos