Related Experiment Videos
Fertilization and pregnancies following intracytoplasmic injection of testicular spermatozoa
A Obruca1, K Mock, W Feichtinger
1Institute for Sterility Treatment, Wien, Austria.
Journal of Assisted Reproduction and Genetics
|October 1, 1995
Abstract
Purpose:
Intracytoplasmic injection (ICSI) with testicular sperm was performed in 16 couples. All men had ductal obstruction and failed previous attempts of epididymal sperm microaspiration.
Methods:
Testis tissue was obtained by excisional biopsies and incubated in HEPES buffered EBSS medium over 24 h at 37 degrees C. Motile sperm (Grade 1 to 2) were recovered in 13 patients and fertilized a total of 62 oozytes. Four pregnancies were achieved.
Results:
One healthy boy and two girls (twin pregnancy) were born.
Conclusions:
The ongoing pregnancies revealed no fetal abnormalities on ultrasound scanning.