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Characterization of 2 aquareovirus proteins
S K Samal1, K Subramanian, B Lupiani
1College of Veterinary Medicine, Maryland Agricultural Experiment Station, University of Maryland, College Park 20742, USA.
Veterinary Research
|January 1, 1995
Summary
Aquareovirus genome segments 10 and 11 encode key proteins. VP7, the major outer capsid protein, is glycosylated with N-linked carbohydrate chains containing galactose residues.
Area of Science:
- Virology
- Molecular Biology
- Protein Chemistry
Background:
- Aquareoviruses are segmented dsRNA viruses with complex protein products.
- Understanding viral protein structure and function is crucial for viral research.
Purpose of the Study:
- To investigate the protein products of aquareovirus genome segments 10 and 11.
- To characterize the major outer capsid protein (VP7) and non-structural proteins (NS29, NS15).
Main Methods:
- Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) to analyze protein molecular weight and electrophoretic mobility.
- Deglycosylation assays to assess protein glycosylation.
- Lectin binding assays to identify specific carbohydrate residues.
- Partial protease mapping to determine protein origins.
Main Results:
- Genome segment 10 encodes VP7, a 35 kDa glycosylated outer capsid protein.
- VP7 possesses short N-linked carbohydrate chains, primarily containing galactose residues.
- Genome segment 11 encodes non-structural proteins NS29 and NS15.
- NS29 and NS15 originate from distinct protein synthesis initiation sites.
Conclusions:
- The VP7 protein of aquareovirus is post-translationally modified by glycosylation.
- The identified non-structural proteins suggest complex regulatory mechanisms in aquareovirus replication.