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Highly sensitive and specific high-performance liquid chromatographic analysis of 7-hydroxywarfarin, a marker for
1Department of Toxicology and Pharmacology, University of Erlangen-Nürnberg, Germany.
Journal of Chromatography. B, Biomedical Applications
|October 20, 1995
Abstract:
The formation of 7-hydroxywarfarin in incubations of (S)-warfarin with human liver microsomes reflects their cytochrome P-4502C9 activity. This paper describes a rapid high-performance liquid chromatographic method for the determination of 7-hydroxywarfarin with high sensitivity, selectivity, and a simple sample clean-up procedure. Separation was achieved with a C18 reversed-phase column and quantification by fluorometric detection. The method employs an internal standard resulting in good accuracy and precision. The limit of detection is 150 fmol for 7-hydroxywarfarin.