Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A gene replacement strategy for engineering nisin

Helen M Dodd1, Nikki Horn1, Catriona J Giffard2

  • 1Department of Genetics and Microbiology, Institute of Food Research, Norwich Research Park, Colney, Norwich NR4 7UA, UK.

Microbiology (Reading, England)
|January 1, 1996
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Discovery of a novel lantibiotic nisin O from Blautia obeum A2-162, isolated from the human gastrointestinal tract.

Microbiology (Reading, England)·2017
Same author

A Novel Tightly Regulated Gene Expression System for the Human Intestinal Symbiont Bacteroides thetaiotaomicron.

Frontiers in microbiology·2016
Same author

Elucidating pathways of Toxoplasma gondii invasion in the gastrointestinal tract: involvement of the tight junction protein occludin.

Microbes and infection·2015
Same author

Cephalosporinases associated with outer membrane vesicles released by Bacteroides spp. protect gut pathogens and commensals against β-lactam antibiotics.

The Journal of antimicrobial chemotherapy·2014
Same author

Luminal microbes promote monocyte-stem cell interactions across a healthy colonic epithelium.

Journal of immunology (Baltimore, Md. : 1950)·2014
Same author

A bacterial homolog of a eukaryotic inositol phosphate signaling enzyme mediates cross-kingdom dialog in the mammalian gut.

Cell reports·2014

Researchers developed a novel lactococcal expression system for producing engineered nisins. This gene replacement strategy enhances nisin yields and allows precise genetic modification of nisA genes.

Area of Science:

  • Microbiology
  • Molecular Biology
  • Biotechnology

Background:

  • Nisin is a bacteriocin with antimicrobial properties.
  • Current methods for producing engineered nisins have limitations.
  • Developing efficient expression systems is crucial for nisin research.

Purpose of the Study:

  • To develop a lactococcal expression system for exclusive production of novel nisins.
  • To enable precise genetic manipulation of the pre-nisin (nisA) gene.
  • To improve yields of engineered nisin molecules.

Main Methods:

  • Constructed a specialized host strain and vectors for nisA gene manipulation.
  • Employed gene replacement technique to substitute wild-type nisA with variant genes.
  • Utilized nisin immunity for direct selection of successful gene replacement strains.

Related Experiment Videos

Main Results:

  • Successfully produced and characterized novel nisins with substitutions (e.g., Dha5A, H27K).
  • Demonstrated exclusive production of engineered nisins via the developed system.
  • Observed enhanced yields of engineered nisins compared to plasmid-complementation systems.

Conclusions:

  • The developed lactococcal expression system enables efficient production of engineered nisins.
  • Gene replacement strategy offers improved yields and precise genetic control over nisin variants.
  • This system facilitates the study and application of novel nisin molecules.