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B-lymphocyte-stimulating polysaccharide from mushroom Phellinus linteus

K S Song1, S M Cho, J H Lee

  • 1Korea Research Institute of Bioscience & Biotechnology. Taejon, Korea.

Chemical & Pharmaceutical Bulletin
|December 1, 1995
PubMed
Summary
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A Phellinus linteus mushroom extract (PLP) significantly boosted antibody production by stimulating B-lymphocytes. Its carbohydrate structure, not protein, is crucial for this immune-enhancing activity.

Area of Science:

  • Immunology
  • Mycology
  • Biochemistry

Background:

  • Phellinus linteus is a medicinal mushroom with potential immunomodulatory properties.
  • Understanding the active components responsible for immune stimulation is crucial for therapeutic development.

Purpose of the Study:

  • To isolate and characterize the active component from Phellinus linteus responsible for stimulating polyclonal antibody production.
  • To investigate the structural requirements of the active component for its B-lymphocyte stimulatory activity.

Main Methods:

  • Hot water extraction of Phellinus linteus mycelia.
  • Purification using ethanol precipitation, DEAE-cellulose, and gel permeation chromatography.
  • Characterization of the purified fraction (PLP) including peptide and carbohydrate content, molecular weight, sugar composition, and amino acid analysis.

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  • Assessment of B-lymphocyte stimulatory activity and structure-activity relationship studies (hydrolysis, periodate, and proteinase K treatment).

    • Main Results:

    • A purified fraction (PLP) was obtained with 1030-fold enrichment, containing 13.2% peptide and 82.5% carbohydrate.
    • PLP is a homogeneous glycoprotein with a molecular weight of 153 kDa, rich in neutral sugars (Gal, Man, Glc) and glucuronic acid.
    • PLP demonstrated potent B-lymphocyte stimulation with a half-maximal concentration of approximately 3 µg/ml.
    • Activity was significantly reduced by acid hydrolysis and periodate oxidation, but unaffected by proteinase K treatment.

    Conclusions:

    • The Phellinus linteus-derived fraction PLP is a potent B-lymphocyte stimulant, primarily acting through its carbohydrate moiety.
    • The structural integrity and carbohydrate composition of PLP are essential for its immunomodulatory activity.
    • These findings highlight the potential of Phellinus linteus as a source of novel immunomodulatory agents.