Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Integrin alpha 2 beta 1 mediates interactions between developing embryonic retinal cells and collagen

A D Bradshaw1, K M McNagny, D B Gervin

  • 1Neuroscience Research Institute, University of California, Santa Barbara 93106, USA.

Development (Cambridge, England)
|November 1, 1995
PubMed
Summary

Integrin alpha 2 beta 1 mediates avian retinal cell adhesion to collagen during development. Its expression and activity decrease with age, suggesting a role in early neuroepithelial cell interactions, not axon extension.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Higher versus lower mean arterial blood pressure after cardiac arrest and resuscitation (MAP-CARE): A protocol for a randomized clinical trial.

Acta anaesthesiologica Scandinavica·2025
Same author

Fever management with or without a temperature control device after out-of-hospital cardiac arrest and resuscitation (TEMP-CARE): A study protocol for a randomized clinical trial.

Acta anaesthesiologica Scandinavica·2025
Same author

Sedation, temperature and pressure after cardiac arrest and resuscitation-The STEPCARE trial: A statistical analysis plan.

Acta anaesthesiologica Scandinavica·2025
Same author

Continuous deep sedation versus minimal sedation after cardiac arrest and resuscitation (SED-CARE): A protocol for a randomized clinical trial.

Acta anaesthesiologica Scandinavica·2025
Same author

Severe hypoxemia and stroke caused by a patent foramen ovale with right-to-left interatrial shunt despite normal right atrial pressures.

International journal of cardiology. Heart & vasculature·2021
Same author

Hedgehog signaling in the airway epithelium of patients with chronic obstructive pulmonary disease.

Scientific reports·2019

Area of Science:

  • Developmental biology
  • Cell biology
  • Neuroscience

Background:

  • The developing nervous system relies on extracellular matrix cues for cell fate, migration, and synapse formation.
  • In the neural retina, collagens support cell adhesion and neurite outgrowth, but specific integrin receptors are unknown.

Purpose of the Study:

  • To identify integrin receptors mediating neuronal responses to collagen in the developing avian retina.
  • To characterize the expression and function of integrin alpha 2 beta 1 in the avian retina.

Main Methods:

  • Immunofluorescence and immunoprecipitation using monoclonal antibody MEP-17 to detect alpha 2 protein.
  • Analysis of alpha 2 expression at different embryonic days (E4, E6, E12).
  • Function-blocking antibodies to assess the role of alpha 2 beta 1 in cell adhesion and process outgrowth on collagen I and IV.

Related Experiment Videos

Main Results:

  • Integrin alpha 2 beta 1 was detected in the developing avian retina, with widespread expression in undifferentiated neuroepithelial cells at E4.
  • Alpha 2 expression decreased in differentiated retinal regions and was absent in retinal ganglion cells at E6.
  • Integrin alpha 2 beta 1 is essential for embryonic retinal cell adhesion and process outgrowth on collagens I and IV, with declining activity in later development.

Conclusions:

  • Integrin alpha 2 beta 1 functions as a collagen receptor in the developing avian retina.
  • This integrin plays a crucial role in neuroepithelial cell interactions with collagen during early retinal development.
  • Its function appears limited to cell adhesion and process extension, rather than axon extension by retinal ganglion cells.