Related Experiment Videos
Calcitonin gene-related peptide (CGRP) in the developing mouse limb
M Bidegain1, B A Roos, E L Hill
1Geriatric Research, Education, and Clinical Center, VA Medical Center, Miami, FL 33125, USA.
Endocrine Research
|November 1, 1995
Summary
Mouse limb buds respond to calcitonin gene-related peptide (CGRP) early in development, even before CGRP is detectable. This neuropeptide influences cyclic AMP (cAMP) levels via CGRP receptors, impacting musculoskeletal development.
Area of Science:
- Developmental Biology
- Neuroendocrinology
- Musculoskeletal Development
Background:
- Neuropeptides play crucial roles during gestation, influencing tissue development.
- Calcitonin gene-related peptide (CGRP) is implicated in mitogenic effects and tissue localization.
Purpose of the Study:
- To investigate the expression and function of CGRP in developing mouse limb buds.
- To understand CGRP's role in early musculoskeletal development.
Main Methods:
- Examined CGRP immunoreactivity (CGRP-ir) in mouse limb buds at different gestational stages (E 11.5 and E 16.5).
- Assessed limb bud responsiveness to CGRP by measuring cyclic AMP (cAMP) levels.
- Utilized a CGRP receptor antagonist (human CGRP8-37) to confirm receptor-mediated effects.
Main Results:
- CGRP-ir was detected in nerve fibers around blood vessels, muscle, and cartilage by E 16.5.
- Limb buds demonstrated responsiveness to CGRP as early as E 11.5.
- CGRP exposure led to a 2-3 fold increase in cAMP levels, mediated by CGRP receptors.
Conclusions:
- Mouse limb buds are sensitive to CGRP during early development, primarily when composed of undifferentiated mesenchyme.
- CGRP expression becomes apparent later, coinciding with cartilage and muscle differentiation.
- CGRP signaling through its receptors is involved in early limb bud development.