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Exercise training generates ascorbate free radical in rat heart

S M Somani1, C M Arroyo

  • 1Department of Pharmacology, Southern Illinois University, School of Medicine, Springfield 62794-9230, USA.

Indian Journal of Physiology and Pharmacology
|October 1, 1995
PubMed
Summary
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Exercise training in old rats generates protective ascorbate radicals in heart tissue, reducing oxidative stress. This finding highlights exercise

Area of Science:

  • Exercise Physiology
  • Biochemistry
  • Gerontology

Background:

  • Exercise can generate free radicals, potentially causing tissue damage.
  • Oxidative stress is a concern in aging tissues.
  • Ascorbate (Vitamin C) plays a role in antioxidant defense.

Purpose of the Study:

  • To investigate the formation of ascorbate free radicals (Asc.-) in heart tissue during exercise training (ET) in aged rats.
  • To determine if ET influences the presence of Asc.- compared to sedentary controls.
  • To explore the potential protective mechanisms of exercise against oxidative damage in the aging heart.

Main Methods:

  • Male Fischer-344 rats (77 weeks old) underwent a 9-week graded exercise training (ET) program on a treadmill.
  • A sedentary control (SC) group was maintained under identical conditions without exercise.

Related Experiment Videos

  • Heart tissues were analyzed for Asc.- using electron paramagnetic resonance (EPR) spectroscopy.

    • Main Results:

    • Ascorbate free radicals (Asc.-) were detected in the heart tissue of ET rats, but not in SC rats.
    • The EPR spectrum of Asc.- exhibited a characteristic doublet with a hyperfine coupling constant of 1.89 Gauss.
    • Exercise training led to increased respiratory exchange ratio, oxygen consumption, and heat production.

    Conclusions:

    • Exercise training promotes the formation of ascorbate radicals in the heart muscle of aged rats.
    • The presence of Asc.- in ET rats suggests a mechanism for reducing free radical toxicity.
    • Exercise training may protect the aging heart against oxidative damage through ascorbate and vitamin E.