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In vivo study of the immunomodulating activity of OM-85 using the plaque-forming cells technique (PFC) in mice
J Mimouni1, A Ramsteiner, R Fontanges
1Université Claude Bernard-Lyon I, Laboratoire de Physiologie 404, Villeurbanne, France.
Abstract:
The activity of increasing oral doses of the bacterial extract OM-85 was determined by using the plaque-forming cells assay in mice. The results showed that the oral administration of OM-85 significantly increased the number of splenic antibody-secreting cells against sheep red blood cells (antigen) compared with controls. A maximal response was reached between 400 and 800 mg/kg body weight, and was reproducible with the different batches of OM-85 used. This study indicates that the oral administration of the immunomodulator allows the activation of splenic B-lymphocytes, probably through the gut-associated lymphoid tissue.
Insights
Oral administration of the bacterial extract OM-85 boosts immune response in mice. This immunomodulator activates splenic B-lymphocytes, enhancing antibody production against foreign antigens.
Area of Science:
- Immunology
- Microbiology
- Pharmacology
Background:
- The bacterial extract OM-85 is known for its immunomodulatory properties.
- Understanding the dose-dependent immune response is crucial for therapeutic applications.
Purpose of the Study:
- To determine the immunomodulatory activity of increasing oral doses of the bacterial extract OM-85.
- To investigate the effect of OM-85 on antibody production and B-lymphocyte activation.
Main Methods:
- The plaque-forming cells assay was employed in mice to assess immune activity.
- Different batches of OM-85 were used to ensure reproducibility.
Main Results:
- Oral administration of OM-85 significantly increased splenic antibody-secreting cells against sheep red blood cells.
- A maximal immune response was observed at doses between 400 and 800 mg/kg body weight.
- The observed effects were reproducible across different batches of OM-85.
Conclusions:
- Oral OM-85 activates splenic B-lymphocytes, likely via the gut-associated lymphoid tissue.
- The bacterial extract demonstrates a dose-dependent immunomodulatory effect.
- OM-85 shows potential as an oral immunomodulator for enhancing immune responses.