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Amyotrophic lateral sclerosis cerebrospinal fluid is not toxic to cultured spinal motor neurons
Y Iwasaki1, K Ikeda, T Shiojima
1Fourth Department of Internal Medicine, Toho University Ohashi Hospital, Tokyo, Japan.
Neurological Research
|October 1, 1995
Abstract:
We have studied an effect of cerebrospinal fluid (CSF) from patients with amyotrophic lateral sclerosis (ALS) on explanted and dissociated ventral spinal cord cultures from 13-day-old rat embryos. CSF samples were obtained from 10 ALS patients, 10 other neurological patients, and 10 non-neurological patients. CSF was added at dilution of 10, 25, and 50%. Neurite length and neuronal survival ratio were not significantly different among these three groups. ALS CSF does not contain a neurotoxic factor on the cultured spinal motor neurons.