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Related Experiment Videos

An ammonium pulse inhibits fluid secretion

K R Lau1, M Donohue, R M Case

  • 1School of Biological Sciences, University of Manchester, G38 Stopford Building, Oxford Road, Manchester M13 9PT, UK.

Pflugers Archiv : European Journal of Physiology
|January 1, 1996
PubMed
Summary

Ammonium chloride (NH4Cl) pulses significantly inhibit salivary gland fluid secretion. Recovery depends on NH4Cl concentration, and the mechanism doesn't involve intracellular pH changes or direct receptor blockade.

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Area of Science:

  • Physiology
  • Pharmacology
  • Cell Biology

Background:

  • Salivary gland fluid secretion is a complex physiological process.
  • Acetylcholine is a known stimulator of salivary secretion.
  • Ammonium chloride pulses are sometimes used to probe cellular mechanisms.

Purpose of the Study:

  • To investigate the effect of ammonium chloride (NH4Cl) pulses on acetylcholine-evoked fluid secretion in perfused mandibular salivary glands.
  • To elucidate the underlying mechanisms of NH4Cl-induced inhibition of salivary secretion.

Main Methods:

  • Perfused rat mandibular salivary glands were exposed to 5-min pulses of varying NH4Cl concentrations (0.5 mM and 20 mM).
  • Fluid secretion evoked by acetylcholine was measured.
  • Intracellular pH and intracellular calcium release were monitored.

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Main Results:

  • NH4Cl pulses almost totally inhibited acetylcholine-evoked fluid secretion.
  • Recovery from 0.5 mM NH4Cl was rapid (within 10 min), while recovery from 20 mM NH4Cl was incomplete after 30 min.
  • Inhibition was not due to intracellular acidosis or impaired intracellular calcium release by acetylcholine.

Conclusions:

  • Ammonium chloride pulses significantly inhibit salivary gland fluid secretion.
  • The inhibitory mechanism does not involve alterations in intracellular pH or direct interference with acetylcholine receptor signaling.
  • Caution is advised when interpreting data from ammonium pulse experiments in salivary gland research.