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Analysis of glucan synthesis by Streptococcus mutans
H K Kuramitsu1, M Smorawinska, Y J Nakano
1Department of Oral Biology, State University of New York, Buffalo, USA.
Developments in Biological Standardization
|January 1, 1995
Abstract:
Glucan synthesis by Streptococcus mutans GS-5 has been analyzed by examining the regulation of expression and structure-function relationships of the glucosyltransferases. Primer extension analysis of the gtf genes has identified the putative promoter sequences for these genes and indicated that the -10 sequences are similar to the Escherichia coli consensus sequence. Site-directed mutagenesis as well as deletion analysis of the enzymes have identified amino acid residues as well as functional domains which play important roles in glucan synthesis.