Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Polysaccharide expression in Lactococci

K P Dierksen1, W Ebel, J Marks

  • 1Department of Microbiology, Oregon State University Corvallis, USA.

Developments in Biological Standardization
|January 1, 1995
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Evidence for X(3872)-->psi(2S)gamma in B(+/-)-->X(3872)K(+/-) decays and a study of B-->cc[over ]gammaK.

Physical review letters·2009
Same author

Measurement of the e;{+}e;{-}-->bb[over ] Cross Section between sqrt[s]=10.54 and 11.20 GeV.

Physical review letters·2009
Same author

Searches for B meson decays to phiphi, phirho, phif_{0}(980), and f_{0}(980)f_{0}(980) final states.

Physical review letters·2008
Same author

Measurement of the branching fractions of B-->D**(l) nu(l) decays in events tagged by a fully reconstructed B meson.

Physical review letters·2008
Same author

Observation and polarization measurements of B+/- -->phiK1 +/- and B +/- -->phiK2 *+/-.

Physical review letters·2008
Same author

Measurement of the CP asymmetry in b-->sgamma using a sum of exclusive final states.

Physical review letters·2008

Researchers identified key regulators of polysaccharide synthesis in Lactococcus lactis using novel vectors and genetic complementation. A conserved protease, Lon, appears to negatively regulate polysaccharide production in this bacterium.

Area of Science:

  • Microbiology
  • Molecular Biology
  • Bacteriology

Background:

  • Lactococcus lactis is known to produce polysaccharides under specific environmental conditions.
  • Understanding the regulation of polysaccharide synthesis is crucial for controlling its production.
  • Identifying genetic regulators is key to manipulating polysaccharide biosynthesis pathways.

Purpose of the Study:

  • To identify and characterize the regulators involved in polysaccharide synthesis in Lactococcus lactis.
  • To develop novel genetic tools for studying gene expression in Lactococcus lactis.
  • To investigate the role of conserved proteases in polysaccharide production.

Main Methods:

  • Development of two novel lactococcal vectors with promoterless reporter genes for identifying chromosomal insertions affecting polysaccharide expression.

Related Experiment Videos

  • Genetic complementation using lactococcal genomic libraries to identify genomic activities influencing capsule expression in Escherichia coli.
  • Immunological detection using antiserum specific to Lon protease to identify related proteins in Lactococcus lactis.

    • Main Results:

    • Novel lactococcal vectors successfully selected for chromosomal insertions impacting polysaccharide expression.
    • Genetic complementation identified two classes of lactococcal genomic activities affecting capsule expression in E. coli.
    • A protein in Lactococcus lactis, similar in size to E. coli Lon protease, was detected; its presence correlated inversely with polysaccharide inducibility.

    Conclusions:

    • The study successfully identified potential regulators of polysaccharide synthesis in Lactococcus lactis using multiple genetic and molecular approaches.
    • The findings suggest that Lon protease, a conserved protease, may act as a negative regulator of polysaccharide synthesis in Lactococcus lactis.
    • The developed lactococcal vectors provide valuable tools for future genetic studies of polysaccharide production in this bacterium.