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Updated: Aug 18, 2026

Preparation of Quality Inositol Pyrophosphates
Published on: September 3, 2011
Identification and chromosomal mapping of the mouse inositol polyphosphate 1-phosphatase gene
1Laboratory of Genetic Disease Research, National Institutes of Health, Bethesda, Maryland 20892-4470, USA.
Abstract:
A mouse inositol polyphosphate 1-phosphatase (Inpp1) cDNA fragment (348 bp) was amplified by means of the polymerase chain reaction using a mouse cDNA library as template with primers designed from published human and bovine cDNA sequences. We isolated a 1623-bp full-length Inpp1 cDNA from a mouse brain cDNA library using this amplified cDNA fragment as probe. Amino acid sequences of mouse, human, and bovine inositol polyphosphate 1-phosphatase are highly conserved. Northern blot analysis shows a major transcript of 1.65-kb mRNA and several higher molecular weight mRNAs that are expressed in a variety of mouse tissues. Utilizing the Jackson Lab backcross DNA panel map service, we mapped Inpp1 to chromosome 1, 1.06 cM proximal to Ctla4.
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