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Purification of a baculovirus-expressed hepatitis E virus structural protein and utility in an enzyme-linked
1Viral and Rickettsial Diseases Program, Naval Medical Research Institute, Bethesda, Maryland, USA.
Journal of Clinical Microbiology
|December 1, 1995
Abstract:
We report on the purification of the full-length structural protein encoded by open reading frame 2 (ORF-2) of hepatitis E virus. The ORF-2 protein, expressed in Sf9 cells by using a recombinant baculovirus vector system, was successfully purified to homogeneity. Gel electrophoresis of the purified ORF-2 protein showed a single polypeptide of 75 kDa by Coomassie blue staining and by Western blot (immunoblot) analysis. We demonstrated that the partially purified ORF-2 protein could be used successfully in a sensitive and specific enzyme-linked immunosorbent assay for the detection of antibodies to hepatitis E virus.