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Related Experiment Videos

Hearing loss and trace elements Fe2+ and Zn2+ in the perilymph

M Xu1, Y Fan, Z Gao

  • 1Department of Otolaryngology, Second Affiliated Hospital, Xi'an Medical University, China.

ORL; Journal for Oto-Rhino-Laryngology and Its Related Specialties
|September 1, 1995
PubMed
Summary

Sea buckthorn oil prevents gentamicin-induced hearing loss by modulating iron and zinc levels in the inner ear. This study highlights the role of perilymph

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Area of Science:

  • Ototoxicity research
  • Inner ear physiology
  • Trace element analysis

Background:

  • Gentamicin (GM) is an antibiotic known to cause ototoxicity, leading to hearing loss.
  • Alterations in metal ion concentrations, particularly iron (Fe2+) and zinc (Zn2+), are implicated in cochlear damage.
  • Understanding these biochemical changes is crucial for developing protective strategies.

Purpose of the Study:

  • To investigate the effects of gentamicin on Fe2+ and Zn2+ levels in guinea pig perilymph, cerebrospinal fluid (CSF), serum, and hair.
  • To evaluate the potential of sea buckthorn oil in preventing or treating gentamicin-induced ototoxicity.
  • To correlate changes in metal ion concentrations with cochlear pathology.

Main Methods:

  • Guinea pigs were injected with gentamicin.

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  • Fe2+ and Zn2+ concentrations were measured in perilymph, CSF, serum, and hair samples.
  • Sea buckthorn oil was administered for prevention and treatment protocols.
  • Comparative analysis of metal ion levels between control and experimental groups.

    • Main Results:

    • Gentamicin injection increased Fe2+ in perilymph and hair, while Zn2+ increased in perilymph and CSF.
    • Sea buckthorn oil administration reduced elevated Fe2+ levels and modulated Zn2+ levels, showing a preventive and therapeutic effect.
    • Changes in perilymph metal ion concentrations were more significant indicators of cochlear damage than those in CSF or serum.

    Conclusions:

    • The ototoxic effects of gentamicin are associated with increased Fe2+ levels in the perilymph.
    • Elevated Zn2+ levels appear to be a compensatory response to gentamicin exposure.
    • Sea buckthorn oil demonstrates efficacy in preventing gentamicin-induced ototoxicity, suggesting its therapeutic potential for hearing loss.