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Effect of penicillin G on the electroporation of Rhodococcus rhodochrous CF222
M Sunairi1, N Iwabuchi, K Murakami
1Department of Applied Biological Science, College of Agriculture and Veterinary Medicine, Nihon University, Kanagawa, Japan.
Letters in Applied Microbiology
|January 1, 1996
Abstract:
Suitable conditions for the introduction of bacteriophage DNA into cells of Rhodococcus rhodochrous CF222 by electroporation were established, and penicillin G was found to enhance the transfection frequency. When conditions optimal for the parental strain were applied to its colony-morphological mutants, different transfection frequencies were observed. Penicillin G enhanced the transfection frequency of smooth and mucoidal mutants but not of rough mutants.