Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Parallel-stranded duplex DNA containing dA.dU base pairs

I Förtsch1, H Fritzsche, E Birch-Hirschfeld

  • 1Institute of Molecular Biology, Friedrich-Schiller University, Jena, Germany.

Biopolymers
|February 1, 1996
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

FKBP5 expression is related to risky foraging in house sparrows.

Hormones and behavior·2026
Same author

Divergent climate impacts despite similar response to temperature in a widespread aerial insectivore.

bioRxiv : the preprint server for biology·2025
Same author

Differentially spliced mitochondrial CYP419A1 contributes to ethiprole resistance in Nilaparvata lugens.

Insect biochemistry and molecular biology·2025
Same author

Screening H3 Histone Acetylation in a Wild Bird, the House Sparrow (<i>Passer Domesticus</i>).

Integrative organismal biology (Oxford, England)·2024
Same author

Thoracic impedance pneumography in propofol-sedated patients undergoing percutaneous endoscopic gastrostomy (PEG) placement in gastrointestinal endoscopy: A prospective, randomized trial.

Journal of clinical anesthesia·2024
Same author

Reconstruction of the Corticospinal Tract in Patients with Motor-Eloquent High-Grade Gliomas Using Multilevel Fiber Tractography Combined with Functional Motor Cortex Mapping.

AJNR. American journal of neuroradiology·2023
Same journal

Untreated Rosehip Powder/Poly(Lactic Acid)/Poly(3-Hydroxybutyrate-Co-4-Hydroxybutyrate) Electrospun Mats for Wound Dressing Applications.

Biopolymers·2026
Same journal

Synthesis, Characterization, and Antidiabetic Evaluation of Sequence-Modified Liraglutide Analogs in a Drosophila melanogaster Model.

Biopolymers·2026
Same journal

Fabrication of an Antibacterial Alginate/Chitosan Hydrogel Dressing Loaded With CuO Nanoparticles for Wound Dressing Applications.

Biopolymers·2026
Same journal

Effect of Chitosan-Alginate Polyelectrolyte Complex Formation and Multilayer Polymer Configuration on the Characteristics of 3D-Printed Metronidazole-Loaded Periodontal Films.

Biopolymers·2026
Same journal

Phenolic Grafting of Oxidized Cellulose Nanofibers Using Ferulic Acid: Structural and Antioxidant Analysis Toward Bioactive Nanomaterials.

Biopolymers·2026
Same journal

Detection of a Target Nucleic Acid by Ligation-Assisted Fluorescence Enhancement of a Peptide Nucleic Acid (PNA) Twin Probe via Disulfide Binding.

Biopolymers·2026
See all related articles

Deoxyuridine (dU) can replace deoxythymine (dT) in parallel-stranded DNA (ps-DNA) duplexes, forming stable structures. However, dU substitution slightly destabilizes the ps-helix compared to dT, with similar effects observed in antiparallel-stranded DNA.

Area of Science:

  • Molecular Biology
  • Biophysics
  • Structural Biology

Background:

  • DNA can form parallel-stranded (ps) duplexes with dA.dU base pairing.
  • Previous studies established ps-DNA formation with dA.dT base pairs.

Purpose of the Study:

  • To characterize the properties of ps-DNA duplexes containing dA.dU base pairs.
  • To compare the stability and structure of dA.dU ps-DNA with dA.dT ps-DNA and antiparallel-stranded (aps) DNA.

Main Methods:

  • Spectroscopic analysis (UV, CD, IR, fluorescence)
  • Nuclease sensitivity assays
  • Thermodynamic analysis (melting temperature, Tm)
  • Force field minimization for structural analysis

Main Results:

Related Experiment Videos

  • Total replacement of deoxythymine (dT) with deoxyuridine (dU) destabilized the ps-helix by -13°C.
  • The dA.dU ps-helix melted 14°C lower than the corresponding aps-helix.
  • Helical parameters showed no significant differences between dT and dU containing DNA.

Conclusions:

  • dA.dU base pairing in ps-DNA is feasible but results in a less stable helix compared to dA.dT.
  • The destabilization effect of dU substitution is consistent across both ps and aps DNA structures.