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Related Experiment Videos

Skin equivalent produced with human collagen

F A Auger1, C A López Valle, R Guignard

  • 1Laboratoire de Recherche des Grands Brûlés/LOEX, Saint-Sacrement Hospital, Quebec, Canada.

In Vitro Cellular & Developmental Biology. Animal
|June 1, 1995
PubMed
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Human skin equivalents (SE) using human collagen showed better structural integrity and delayed dermal degradation compared to bovine collagen SE. This highlights human collagen

Area of Science:

  • Tissue engineering
  • Dermatology
  • Biomaterials science

Background:

  • Cultured skin equivalents (SE) are engineered tissues using human keratinocytes on dermal equivalents (DE).
  • Previous work established an anchorage method to prevent dermal equivalent surface reduction due to fibroblast contraction.
  • A novel anchored human SE was developed for improved histological and biological properties.

Purpose of the Study:

  • To compare the effects of different collagen origins (bovine vs. human) on the in vitro development of anchored skin equivalents.
  • To evaluate the structural and biological characteristics of skin equivalents made with bovine collagen, human collagen, and human collagen with additional matrix components.

Main Methods:

  • Human keratinocytes were seeded on three types of anchored dermal equivalents: bovine SE (bSE), human SE (hSE), and human SE with additional dermal matrix (hSE+).

Related Experiment Videos

  • Comparative immunohistological analysis was performed.
  • Levels of transglutaminase, ceramides, keratin 1, and gelatinase activity were quantified.

    • Main Results:

    • Human SE (hSE and hSE+) exhibited good structural organization and basement membrane component deposition.
    • Elevated transglutaminase, ceramides, and keratin 1 were observed in epidermal layers of all SE cultured at the air-liquid interface.
    • Bovine SE (bSE) showed higher 92 kDa gelatinase activity compared to hSE.
    • Using human collagens delayed dermal layer degradation in culture compared to bovine collagen.

    Conclusions:

    • Anchored human skin equivalents (hSE and hSE+) demonstrate superior structural organization.
    • Human collagen-based dermal equivalents provide enhanced stability and reduced degradation in vitro compared to bovine collagen.
    • The origin of collagen significantly impacts the in vitro evolution and stability of bioengineered skin tissues.