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Electron spectroscopic imaging of encapsidated DNA in vaccinia virus
G Harauz1, D H Evans, D R Beniac
1Department of Molecular Biology and Genetics, University of Guelph, Canada.
Canadian Journal of Microbiology
|October 1, 1995
Abstract:
We have used electron spectroscopic imaging to locate the phosphorus in vaccinia DNA in situ in unstained, ultrathin sections of virions. The phosphorus of the DNA backbone appeared to form a halo on the core periphery surrounding a phosphorus-impoverished central element. These results constrain models for how DNA could be packaged into mature vaccinia particles.