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Mobile genetic elements in mycobacteria

J W Dale1

  • 1Molecular Microbiology Group, University of Surrey, Guildford, UK.

The European Respiratory Journal. Supplement
|September 1, 1995
PubMed
Summary
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Mobile genetic elements like plasmids and transposons are crucial in mycobacteriology research. These elements aid in developing vaccines, diagnostics, and gene manipulation for mycobacteria.

Area of Science:

  • Microbiology
  • Molecular Biology
  • Genetics

Background:

  • Mobile genetic elements (MGEs) such as plasmids, bacteriophages, insertion sequences, and transposons are integral to mycobacterial research.
  • These elements have diverse roles in both fundamental and applied scientific investigations within mycobacteriology.

Purpose of the Study:

  • To review the molecular biology and significance of plasmids and insertion sequences/transposons in mycobacteria.
  • To highlight their applications as cloning vectors and in transposon mutagenesis.

Main Methods:

  • Literature review focusing on plasmids, insertion sequences, and transposons in mycobacteria.
  • Analysis of their roles in vaccine development, diagnostics, and genetic studies.

Main Results:

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  • Plasmids and bacteriophages are utilized as cloning vectors, particularly for recombinant vaccines like bacille Calmette-Guérin (BCG).
  • Insertion sequences are valuable for diagnostic and epidemiological studies, while transposon mutagenesis facilitates gene inactivation and marking.
  • Specific elements like IS6110/IS986 in M. tuberculosis and the IS900 family in M. avium are highlighted.

Conclusions:

  • MGEs are indispensable tools in mycobacteriology, offering versatile applications in research and biotechnology.
  • Understanding these elements enhances our ability to manipulate and study mycobacterial species effectively.