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Microbial production of specifically ring-13C-labelled 4-hydroxybenzoic acid
R Müller1, A Wagener, K Schmidt
1Institut für Pharmazeutische Biologie, Rheinische Friedrich-Wilhelms-Universität Bonn, Germany.
Applied Microbiology and Biotechnology
|November 1, 1995
Abstract:
When transformed with a recombinant vector carrying the ubiC gene (encoding chorismate pyruvate-lyase, EC 4.1.3.27) the triple mutant (Phe-, Trp-, Tyr-) Klebsiella pneumoniae 62-1 excretes 4-hydroxybenzoic acid instead of chorismic acid. The recombinant strain can be used to produce in high yield specifically ring-labelled 4-hydroxybenzoic acid from isotopically labelled glucose.