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[Mechanisms of chorionic gonadotropin action on splenocytes, suppressed in their humoral immune response]
Chorionic gonadotropin (CG) used at 10 and 50 IU/ml concentrations was introduced into a short-term macroculture of splenocytes of CBA mice suppressors fractionated on a nylon wool column. Regulatory effects of CG were evaluated in a syngeneic transfer system by the degree of inhibition of a humoral immune response in recipients. It was found that CG (50 IU/ml) inhibits the functional activity of splenocyte suppressors. The observed effect of the hormone was blocked by introduction into the incubation medium of the inhibitor, inositol-1-monophosphatase (lithium), and did not depend on cyclooxygenase (voltaren) or potential-independent Ca(2+)-channel (verapamil) inhibitors. Determination of the intracellular cAMP level by radioimmunoassay revealed a reliable increase of this secondary messenger in splenocyte suppressors during their incubation with CG. The ability of CG (50 IU/ml) to inhibit the functional activity of intact T-lymphocytes (but not B-cells) was established. The suppressive hormone activity was also followed by cAMP level increase in target cells. These findings suggest that the mechanism of a transmembrane transfer of the CG signal in splenocyte suppressors is related to the adenylate cyclase system and, probably, to phosphoinositide metabolism products.
Chorionic gonadotropin (CG) used at 10 and 50 IU/ml concentrations was introduced into a short-term macroculture of splenocytes of CBA mice suppressors fractionated on a nylon wool column. Regulatory effects of CG were evaluated in a syngeneic transfer system by the degree of inhibition of a humoral immune response in recipients. It was found that CG (50 IU/ml) inhibits the functional activity of splenocyte suppressors. The observed effect of the hormone was blocked by introduction into the incubation medium of the inhibitor, inositol-1-monophosphatase (lithium), and did not depend on cyclooxygenase (voltaren) or potential-independent Ca(2+)-channel (verapamil) inhibitors. Determination of the intracellular cAMP level by radioimmunoassay revealed a reliable increase of this secondary messenger in splenocyte suppressors during their incubation with CG. The ability of CG (50 IU/ml) to inhibit the functional activity of intact T-lymphocytes (but not B-cells) was established. The suppressive hormone activity was also followed by cAMP level increase in target cells. These findings suggest that the mechanism of a transmembrane transfer of the CG signal in splenocyte suppressors is related to the adenylate cyclase system and, probably, to phosphoinositide metabolism products.